Team:Warsaw/Calendar-Main/12 June 2008

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Blue/white and rifampicin test

Michał L., Ewa, Marcin:

Blue colonies of each transformant strain were counted.
Results (% of blue colonies):
pMPMT5 100%
pMPMT5-AID 98%
pMPMT5-AIDT7 76%
pMPMT5-AID+T7 0.2%

Results of rifampicin test
StrainInducer / repressorNumber of colonies on rifampicin
Top10none3
Top10 pMPM-AID0.1% Arabinose63
Top10 pMPM-AIDnone9
Top10 pMPM-A+T130.1% Arabinose25
Top10 pMPM-A+T13none7
Top10 pMPM-AT60.1% Arabinose127
Top10 pMPM-AT6none14