Team:Warsaw/Calendar-Main/26 June 2008

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Preparation of constructs with OmpA protein fusions

Michał K.

Repetition of PCRs, gel-out isolations and digests from 17 June.

Polymerase Chain Ligation (PCL)

Michał L., Ewa, Marcin

ProductTemplatesPrimersProduct length
Alpha-A fusionAlpha-linker + linker-AAlphaL+SacI + AP+NotI 1500 bp
Omega-A fusionOmega-linker + linker-AOmegaL+SacI + AP+NotI 1440 bp


PCL program for fusion A with alpha and omega
TemperatureTime
94°C4:00
94°C0:3028 cycles
48°C-62°C0:45
68°C2:30
68°C10:00
4°Cinfinite

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