Team:Warsaw/Calendar-Main/2 July 2008

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Preparation of constructs with OmpA protein fusions

Paweł

  1. Clean-up of overnight digest.
  2. Ligation of OmpA_alfa and pET15b.
  3. Ligation of OmpA_omega and pET15b.
  4. Transformation of E. coli TOP10 strain with ligation products.
  5. Transformants plating on LB + ampicillin.

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