Team:Warsaw/Calendar-Main/29 May 2008

From 2008.igem.org

Revision as of 14:00, 11 October 2008 by Emilia (Talk | contribs)

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Rifampicin test

Michał L., Ewa, Marcin

  1. Added 50 μL E. coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB.
  2. Added 50 μL E. coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB + 0.02% arabinose.
  3. Added 50 μL E. coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB.
  4. Added 50 μL E. coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB + 1 mM IPTG.
  5. Waiting 5 hours.
  6. 1 ml (2x) from p.1, p.2, p.3, p.4 (LB + rifampicin).

Piotr

OD measurement of above cultures.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/29_May_2008"