Cloning of protein A DNA to OmpA constructs

1. Colony PCR on colonies from plates with transformations pACYC177+OmpA_A_omega and pACYC177+OmpA_A_alpha. Primers used: AL+SacI and AP+NotI
2. Confirmed transformant colonies (found only on pACYC177+OmpA_A_alpha plate) inoculated to liquid LB with kanamycin.

Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA
Paweł

1. Ligation transformed into TOP10 strain and plated on LB+kanamycin

Cloning of protein Z DNA to OmpA constructs

Michał K.

1. 4 colonies from transformation pACYC177+OmpA_Z_alpha were inoculated to liquid LB broth with kanamycin

Cloning of protein Z DNA to OmpA constructs
Michał K.

1. Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_Z_alpha).
2. Control digest of isolated plasmids with BamHI and SacI (we found 4 good clones).