Team:Warsaw/Calendar-Main/18 July 2008

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Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA

Paweł

Ligation transformed into TOP10 strain and plated on LB + ampicillin.

Cloning of protein Z DNA to OmpA constructs

Michał K.

Colonies from transformation pACYC177+OmpA_Z_alpha were inoculated to liquid LB broth with kanamycin.