Sequencing confirms that we have obtained construct with omega-A fusion, but A in our constructs turned out to be trunctated and contain only one from two highly similar domains.
Checking if omp_omega_A_alpha gives ampicillin resistance
Piotr
Inoculation of omp_omega_A_alpha into various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75, 1 mmol/mL
Checking the expression of omp_omega_A_alpha and omp_A_alpha
Piotr
Inoculation of omp_omega_A_alpha and omp_A_alpha with inductor (0,5 mmol/mL IPTG) and negative control without IPTG.