Alberta NINT/8 July 2008

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lab work (SD): 

            Transformation results:
                                    ara +               ara -
            K102022                 ++++                ++++
            K102032                  +++                 +++
            K102032 + K102011          -                  ++
            K102032 + K102034         ++                  ++
            K102032 + K102035          -                   +
            K102032 + K102036          +                   +

            where ++++ is the most blue and + is the least blue. - indicates white colonies.

lab work (JD): 

                 Collected new quantitative data of K102003/E+P, K102006/E+P and newly cut pSB4A5
                 Carried out ligation of K102003+pSB4A5 and K102006+pSB4A5
                 Collected new pSB4A5 from iGEM binder
                 Transformed XL1-B cells with ligations
                 Transformed DB3.1 cells with new pSB4A5 part
                 Plated cells on LB +Amp50 plates
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