Alberta NINT/21 August 2008

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lab work (SD): 

            Ligated TA2+(PCR)/B+N (NR) into K102031/B+N (GP).
            Transformed into XL1-B cells and incubated overnight at 37 C.

lab work (JD): 

             Digested mini-prep of cotransformed cells with EcoR1
                   -to run on gel and check if in fact both plasmids inside the cell are low copy 
                    plasmids, bands should be about the same brightness
             Ran on gel and found that the band intensities were not the same.
             UVP0343.jpg
             Analyzed LacZ Assay Data
             Media:Alberta_NINT_LacZ_assay_August_22_08.xls
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