Preparation of alpha-A and omega-A fusions

Michał L., Ewa, Marcin

Still no success. We need to run gradient PCR to find optimal reaction conditions.

Preparation of constructs with OmpA protein fusions

Michał K.

1. PCR on isolated plasmids with OmpaL_N and OmpaP_link primers.
2. Gel electrophoresis (Fig. 1, no proper clones found).

Blue/white and rifampicin test

Michał L., Ewa, Marcin

Sequencing determined that all white colonies from 16 June have intact lacZ gene. Reason of white phenotype unknown. We suppose that it is due to chromosomal mutation. We need another reporter system (not splitted to chromosomal and plasmid part --> GFP? ).