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Team:Warsaw/Calendar-Main/21 August 2008
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Cloning of protein A DNA to GeneArt plasmid
Antoni
Colony
PCR
on colonies from plates with transformations pGeneart+A Primers used:
AP+NotI
and
AL+SacI
.
Confirmed transformant colonies inoculated to liquid LB with ampicillin.
Inoculation to liquid LB with ampicillin: pET15b+OmpA-alpha.