Team:Warsaw/Calendar-Main/27 August 2008

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Cloning of protein Z DNA to pET15b+Omp-alfa plasmid

Antoni

Transformant colonies inoculated to liquid LB with ampicillin.

Cloning of protein A DNA to pET15b+Omp-alfa plasmid

Michał K.

  1. Digest of pGeneart+A and pET15b+Omp-alfa with NdeI and NotI (Orange buffer), pET15b was also dephosphorylated.
    2. .
  2. Gel electrophoresis of digested plasmids and gel-out of proper bands (400 bp for A lane and 6200 bp for pET15b lane).
  3. Ligation of pET15b+alfa and A.
  4. Transformation of E. coli TOP10.
  5. Transformants plating on LB + ampicillin.

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