Cloning of protein Z DNA to pET15b+Omp-alpha plasmid

Antoni

1. Isolation of pET15b+OmpA-alfa plasmids.
2. Control digest of pGeneart+A plasmids with NdeI and NotI (Orange buffer).
3. Gel elctrophoresis. Chioce of proper clone.

Cloning of protein A DNA to pET15b+Omp-alpha plasmid

Michał K.

1. Colony PCR on colonies from plates with transformations pEt15b+A_alpha.
   Primers used: AP+NotI and AL+SacI.
2. Gel electrophoresis.
3. Confirmed transformant colonies inoculated to liquid LB with ampicillin.