Cloning of protein Z DNA to pET15b+Omp-alpha plasmid

Antoni

Transformant colonies inoculated to liquid LB with ampicillin.

Cloning of protein A DNA to pET15b+Omp-alpha plasmid

Michał K.

1. Digest of pGeneart+A and pET15b+Omp-alpha with NdeI and NotI (Orange buffer), pET15b was also dephosphorylated.
2. Gel electrophoresis of digested plasmids and gel-out of proper bands (400 bp for A lane and 6200 bp for pET15b lane).
3. Ligation of pET15b+alpha and A.
4. Transformation of E. coli TOP10.
5. Transformants plating on LB + ampicillin.