Team:Warsaw/Calendar-Main/21 August 2008

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Cloning of protein A DNA to GeneArt plasmid

Antoni

  1. Colony PCR on colonies from plates with transformations pGeneart+A.
    Primers used: AP+NotI and AL+SacI.
  2. Gel electrophoresis.
  3. Confirmed transformant colonies inoculated to liquid LB with ampicillin.
  4. Inoculation to liquid LB with ampicillin: pET15b+OmpA-alpha.

  1. Isolation of plasmids from cultures inocluated on previous day.
  2. Control digest of isolated plasmids with FastBamHI and FastSacI (Fast Digest buffer).
  3. Gel electrophoresis - no proper clones founded.

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