Team:Warsaw/Calendar-Main/25 August 2008

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Cloning of protein A DNA to pET15b+Omp-alpha plasmid

Antoni

  1. Transformation of E. coli TOP10 strain with friday's ligation products.
  2. Transformants plating on LB + ampicillin.

Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omega

Michał K.

Eight colonies from each plate (pACYC177+OmpA_alpha_A_omega and pACYC177+OmpA_alpha_Z_omega) was inoculated to liquid LB with kanamycin.

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