Team:Paris/Notebook/Freezer

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Last number

(check on 11 sept 2008, subject to change by unwritten data on the wiki !! FC)

Last Ligation number used

213

Last PCR number used

154

Last Digestion number used

202

Last Miniprep number used

215

Last Strain number used

215

PCR products

PCR products
Number	Name	Oligo F	Oligo R	Template	Length	Comments	Product Left ?	[DNA]µg/mL	Date
PCR124	pFlgA	O100	O101	-	261pb		yes	0.5	August 7
PCR125	pFlgB	O102	O103	-	261pb		yes	0.5	August 7
PCR126	pFlhB	O108	O109	-	260pb		yes	0.5	August 7
PCR127	FlhDC	O132	O131	MG1655			no		August 8
PCR128	OmpR*	O138	O139	Strain OmpR*	700pb		yes	150	August 8
PCR129	EnvZ*	O126	O127	Strain EnvZ*	1400pb		yes	100	August 8
PCR130	E0240 rbs+	O141	O140	MP143	900pb		no		August 11
PCR131	FlhD rbs-	O132	O133	MG1655	350pb		no		August 11
PCR132	pFlhD rbs-	O130	O131	MG1655	600pb		no		August 11
PCR133	FlhDC + prom	O110	O131	MG1655	1300pb		no		August 11
PCR134	FlhDC + prom	O111	O131	MG1655			no		August 11
PCR135	pFliL	O124	O125	MG1655			yes		August 11
PCR136	pFlhDC	O110	O113	MG1655			yes		August 11
PCR137	pFlhDC	O 111	O 113	MG1655			yes		August 11
PCR138	FlhDC (RBS -)	O 132	O 131	MG 1655			yes		August 15
PCR139	pFlhDC (URI)	O 110	O 113	PCR 130			yes		August 15
PCR140	FlhDC (RBS -)	O 132	O 131	PCR 130			yes		August 15
PCR141	Gene FlhDC + rbs nat	O 134	O 131	MG1655	1000pb		yes		August 26
PCR142	pFliA + rbs nat	O 145	O 144	MG1655	325pb		yes		August 26
PCR143	pFliA sans rbs	O 145	O 146	MG1655	310pb		yes		August 26
PCR144	pFliA + Gene FliA	O 145	O 151	MG1655	1100pb		yes		August 26
PCR145	PCRFliA1	O 148	O 152	MG1655	150pb		yes		August 26
PCR146	PCRFliA2	O 153	O 150	MG1655	670pb		yes		August 26
PCR 147	EnvZ*	O 126	O 127	S 120	1412 bp		yes	150	August 26
PCR 148	OmpR*	O 138	O 139	S 119	789 bp		yes	101	August 26
PCR 149	pFlhDC (Soutourina +RBS)	O 154	O 113	MG1655	450 bp		yes		August 26
PCR 151	pFLiA without nat. rbs	O 145	O 146	MG1655	310 bp		yes	34	August 26
PCR 152	pFliA rbs nat.	O 145	O 144	MG1655	325 bp		yes	19	August 26
PCR 153	pFliA+Gene FliA non-mutated	O 145	O 151	MG1655	1100 bp		yes	39	August 26
PCR 154	Gene FliA mutated (EcoRI/PstI)	O 148	O 150	MG1655	800 bp	154.1, 154.2, 154.3	yes	50	August 26

Ligations: Constructions realised

Plasmids: MiniPrep Products 2008
Number	Plasmid	Strain	Miniprep	Description	Digestion	comment	Date
L100	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S146	MP147	rbs TetR - ECFP	D110 (BV) - D130 (BI)		August 13
L101	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S147	MP148	rbs TetR - GFP tripart	D110 (BV) - D131 (BI)	DO NOT WORK !!!!	August 13
L102	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S123	MP124	Strong rbs - YFP	D129 (BV) - D118 (BI)		August 1
L103	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S124	MP125	Strong rbs - mRFP	D129 (BV) - D122 (BI)		August 1
L104	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S125	MP126	Strong rbs - lasR activator	D129 (BV) - D114 (BI)		August 1
L105	[http://partsregistry.org/Part:J61002 J61002]	S126	MP127	Strong promoter - ECFP	D123 (BV) - D130 (BI)		August 1
L106	[http://partsregistry.org/Part:J61002 J61002]	S127	MP128	Strong promoter - gfp Tripart	D123 (BV) - D131 (BI)		August 1
L107	[http://partsregistry.org/Part:J61002 J61002]	S128	MP129	Strong promoter - ECFP	D103 (BV) - D130 (BI)		August 1
L108	[http://partsregistry.org/Part:J61002 J61002]	S129	MP130	Strong promoter - gfp Tripart	D103 (BV) - D131 (BI)		August 1
L109							August
L110	[http://partsregistry.org/Part:J61002 J61002]	S130	MP131	Medium promoter - gfp Tripart	D124 (BV) - D131 (BI)		August 1
L111	[http://partsregistry.org/Part:J61002 J61002]	S131	MP132	Weak promoter - ECFP	D104 (BV) - D130 (BI)		August 1
L112	[http://partsregistry.org/Part:J61002 J61002]	S132	MP133	Weak promoter - GFP tripart	D104 (BV) - D131 (BI)		August 1
L113							August
L114	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	S148	MP149	AracpBAD - gfp tripart	D126 (BV) - D131 (BI)		August 13
L115	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S133	MP134	pLas - ECFP	D105 (BV) - D130 (BI)		August 1
L116	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S134	MP135	pLas - gfp Tripart	D105 (BV) - D131 (BI)		August 1
L117	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	S135	MP136	yfp - Double Terminator	D125 (FV) - D117 (FI)		August 1
L118	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	S136	MP137	rfp - Double Terminator	D125 (FV) - D121 (FI)		August 1
L119	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	S137	MP138	lasR activator + LVA - Double Terminator	D125 (FV) - D113 (FI)		August 1
L120	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S149	MP150	tetR repressible promoter - ECFP	D106 (BV) - D130 (BI)		August 13
L121	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S138	MP139	pTet - gfp tripart	D106 (BV) - D131 (BI)		August 1
L122	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S150	MP151	RBS-lasI - ECFP	D107 (BV) - D130 (BI)		August 13
L123	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S151	MP152	RBS lasI - gfp tripart	D107 (BV) - D131 (BI)		August 13
L124	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S139	MP140	Strongest RBS - rfp	D102 (BV) - D122 (BI)		August 1
L125	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S140	MP141	Strongest RBS - yfp	D102 (BV) - D118 (BI)		August 1
L126	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S152	MP153	Strongest RBS (1)- LasR activator (+LVA)	D102 (BV) - D114 (BI)		August 13
L128	[http://partsregistry.org/Part:J61002 J61002]	S143	MP144	pFlgA-RFP	D132 (FI) - D136 (FV)		August 12
L129	[http://partsregistry.org/Part:J61002 J61002]	S144	MP145	pFlgB-RFP	D133 (FI) - D136 (FV)		August 12
L130	[http://partsregistry.org/Part:J61002 J61002]	S145	MP146	pFlhB-RFP	D134 (FI) - D136 (FV)	wrong sequencing	August 12
L131	[http://partsregistry.org/Part:J61002 J61002]			pFlhDC-RFP	D136 (FV)- D135 (FI)	wrong sequencing	August 8
L132	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S153	MP154	flhDC	D142(FV) - D139(FI)		August 13
L133	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S154	MP155	OmpR*	D142(FV) - D140(FI)	WT sequence (August 19)	August 13 ; August 19
L134	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S155	MP156	EnvZ*	D142(FV) - D141(FI)	lacks about 300 bp in EnvZ* (August 19)	August 13 August 19
L135	[http://partsregistry.org/Part:pSB3K3 pSB3K3]			pFlgA	D137 (BV) - D133 (BI)	don't work	August 9
L136	[http://partsregistry.org/Part:pSB3K3 pSB3K3]			pFlgB	D137 (BV) - D1343 (BI)	don't work	August 9
L137	[http://partsregistry.org/Part:pSB3K3 pSB3K3]			pFlhB	D137 (BV) - D135 (BI)	don't work	August 9
L138	[http://partsregistry.org/Part:pSB3K3 pSB3K3]	S156	MP157	gfp generator (E0240)	D137(FV) - D138(FI)		August 13
L139	[http://partsregistry.org/Part:pSB3K3 pSB3K3]	S159	MP158	E0240 RBS+	D140 (PstI) - D144 (ES)	seems to have the plasmid without the insert and one with the insert	August 15
L140	[http://partsregistry.org/Part:pSB1A2 pSB1A2]			flhD	D145 (V) - D141 (I)		August 12
L141	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S160	MP159	flhC	D142 (ES) - D145 (ES)	has not been sequenced	August 15
L142	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S161	MP160	flhDC+promotor	D143 (ES) - D145 (ES)	does not have the insert	August 15
L143						didn't work	August 14
L144							August
L145							August
L146							August
L147							August
L148							August
L149							August
L150	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		MP161	pLas + rbs-tetR-gfp tripart	D105 (BV) - D146 (BI)	incorrect clone -> to do again	August 19
L151	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	S162	MP162	rbs-lasR + double terminator	D147 (FI) - D125 (FV)		August 19
L153	[http://partsregistry.org/Part:BBa_J61002 J61002]	S169	MP169	pJ23100-rbs-lasER-dble ter	D123 (BV) - D165 (BI)		August 20
L154	[http://partsregistry.org/Part:BBa_J61002 J61002]	S170	MP170	pJ23101-rbs-lasER-dble ter	D103 (BV) - D165 (BI)		August 20
L155				pJ23101-gfp generator	D164(FV) - D163 (FI)		August 22
L156				pTet-gfp generator	D161 (FV) - D163 (FI)		August 22
L157				tetR-rbs (B0015)	D125 (BV) - D162 (BI)		August 23
L158				rbs-tetR-gfp generator	D110(BV) - D131 (BI)		August 23
L159				rbs-lasI-dbl ter	D125 (FV) - D109 (FI)		August
L160							August
L161							August
L162					D		August
L163							August
L164							August
L165							August
L166	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		MP172	RBS(B0032) - tetR			August 25
L167	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		MP171	gfp generator(E0240) - pTet			August 25
L168							September
L169							September
L170							September
L171							September
L172							September
L173							September
L174							September
L175							September
L176					D125 (v) + D162 (i)		September
L177							September
L178							September
L179							September
L180					D166 (v) + D109.2 (i)		September
L181					D199 (v) + D109.2 (i)		September
L182					D187 (v) + D109.2 (i)		September
L183							September
L184							September
L185							September
L186					D179 (v) + D205 (i)		September
L187 ("L1")							September
L188 ("L2")							September
L189 ("L3")							September
L190 ("L4")					D199 (v) + D (i)		September
L191 ("L5")							September
L192 ("L6")							September
L193 ("L7")							September
L194 ("L8")							September
L195 ("L9")					D187 (v) + D194 (i)		September
L196 ("L10")							September
L							September
L							September
L							September

Plasmids: MiniPrep Products

Plasmids: MiniPrep Products 2008
Number	Name	Plamsid	Biobricks	Description	Products in use/comments	Conc. ug/mL	Date
MP100	[http://partsregistry.org/Part:BBa_B0034 B0034 ]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strongest RBS (Efficiency = 1)			July 26
MP101.1	[http://partsregistry.org/Part:BBa_J23101 J23101]	[http://partsregistry.org/Part:BBa_J61002 J61002 ]		Strongest Constitutive promoter	July 26	333	July 22, July 23, July 26
MP101.2						finished
MP101.4						200
MP102	[http://partsregistry.org/Part:BBa_J23109 J23109]	[http://partsregistry.org/Part:BBa_J61002 J61002]		Weak Constitutive promoter	July 26		July 22, July 26
MP103	[http://partsregistry.org/Part:BBa_R0079 R0079]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		promoter pLas (lasr & PAI regulated)	July 26		July 23, July 26
MP104.1	[http://partsregistry.org/Part:BBa_R0040 R0040]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		tetR repressible promoter	July 26	145	July 23, July 26
MP104.3						147
MP104.4						51
MP105.1	[http://partsregistry.org/Part:BBa_S03154 S03154]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		B0034(rbs) ->LasI		34	July 26
MP105.2	[http://partsregistry.org/Part:BBa_S03154 S03154]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		B0034(rbs) ->LasI		31	July 26
MP106.1	[http://partsregistry.org/Part:BBa_S03879 S03879]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		B0034(rbs) -> TetR		80	August 24
MP106.3	[http://partsregistry.org/Part:BBa_S03879 S03879]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		B0034(rbs) -> TetR		40	August 24
MP107	[http://partsregistry.org/Part:BBa_C0079 C0079]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]		lasR activator with LVA Tag			July 23
MP108	[http://partsregistry.org/Part:BBa_C0179 C0179]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		lasR activator	July 26		July 22, July 23, July 26
MP109	[http://partsregistry.org/Part:BBa_E0030 E0030]	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]		YFP			July 26
MP110	[http://partsregistry.org/Part:BBa_E0040 E0040]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		GFP	July 26		July 23, July 26
MP111	[http://partsregistry.org/Part:BBa_E1010 E1010 ]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]		mRFP	July 26		July 23, July 26
MP112	OmpR M57I						July 23
MP113	EnvZ I281S						July 23
MP114.1	[http://partsregistry.org/Part:BBa_C0040 C0040]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		tetR repressor		173	July 23
MP114.2	[http://partsregistry.org/Part:BBa_C0040 C0040]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		tetR repressor		263	July 23
MP115	[http://partsregistry.org/Part:pSB1A3 pSB1A3]						July 23
MP116	[http://partsregistry.org/Part:BBa_J23100 J23100]	[http://partsregistry.org/Part:BBa_J61002 J61002]		Strong constitutive promoter in J61002			July 26
MP117	[http://partsregistry.org/Part:BBa_J23107 J23107]	[http://partsregistry.org/Part:BBa_J61002 J61002]		Medium constitutive promoter in J61002	July 26		July 24, July 26
MP118.1	[http://partsregistry.org/Part:BBa_B0015 B0015]	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]		Double terminator	July 26	220	July 24, July 26
MP118.3	[http://partsregistry.org/Part:BBa_B0015 B0015]	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]		Double terminator	July 26	187	July 24, July 26
MP119.1	[http://partsregistry.org/Part:BBa_I0500 I0500]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]		AraC pBAD	July 26	42	July 24, July 26
MP119.2						26
MP119.3						39
MP120	[http://partsregistry.org/Part:BBa_B0030 B0030]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strong RBS (Efficiency = 0,6)			July 26
MP121	[http://partsregistry.org/Part:BBa_E0422 E0422]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		ECFP (RBS+LVA+Double Term)	July 26		July 24, July 26
MP122	[http://partsregistry.org/Part:BBa_E0840 E0840]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		gfp tri-part; strong rbs			July 26
MP123		[http://partsregistry.org/Part:BBa_J61002 J61002]					August 5
MP124	L102	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strong rbs - YFP D129 (BV) - D118 (BI)			August 1
MP125	L103	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strong rbs - mRFP D129 (BV) - D122 (BI)			August 1
MP126	L104	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strong rbs - lasR activator D129 (BV) - D114 (BI)			August 1
MP127	L105	[http://partsregistry.org/Part:J61002 J61002]		Strong promoter - ECFP D123 (BV) - D130 (BI)			August 1
MP128	L106	[http://partsregistry.org/Part:J61002 J61002]		Strong promoter - gfp Tripart D123 (BV) - D131 (BI)			August 1
MP129	L107	[http://partsregistry.org/Part:J61002 J61002]		Strong promoter - ECFP D103 (BV) - D130 (BI)			August 1
MP130	L108	[http://partsregistry.org/Part:J61002 J61002]		Strong promoter - gfp Tripart D103 (BV) - D131 (BI)			August 1
MP131	L110	[http://partsregistry.org/Part:J61002 J61002]		Medium promoter - gfp Tripart D124 (BV) - D131 (BI)			August 1
MP132	L111	[http://partsregistry.org/Part:J61002 J61002]		Weak promoter - ECFP D104 (BV) - D130 (BI)			August 1
MP133	L112	[http://partsregistry.org/Part:J61002 J61002]		Weak promoter - GFP tripart D104 (BV) - D131 (BI)			August 1
MP134	L115	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		pLas - ECFP D105 (BV) - D130 (BI)			August 1
MP135	L116	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		pLas - gfp Tripart D105 (BV) - D131 (BI)			August 1
MP136	L117	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]		yfp - Double Terminator D125 (FV) - D117 (FI)			August 1
MP137	L118	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]		rfp - Double Terminator D125 (FV) - D121 (FI)			August 1
MP138	L119	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]		lasR activator + LVA - Double Terminator D125 (FV) - D113 (FI)			August 1
MP139	L121	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		pTet - gfp tripart D106 (BV) - D131 (BI)			August 1
MP140	L124	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strongest RBS - rfp D102 (BV) - D122 (BI)			August 1
MP141	L125	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strongest RBS - yfp D102 (BV) - D118 (BI)			August 1
MP142		[http://partsregistry.org/Part:pSB3K3 pSB3K3]		pSB3K3 (low to medium copy plasmid with p15A ORI / kanamycine resistance)			August 8
MP143.1	[http://partsregistry.org/Part:BBa_E0240 E0240]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		gfp generator		138	August 8
MP143.2	[http://partsregistry.org/Part:BBa_E0240 E0240]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		gfp generator		150	August 8
MidiP144	L128	[http://partsregistry.org/Part:BBa_J61002 J61002]		pFlgA-RFP D132 (FI) - D136 (FV)		538	August 12
MP144.2						166
MP144.3						163
MP144.4						154
MP145.1	L129	[http://partsregistry.org/Part:BBa_J61002 J61002]		pFlgB-RFP D133 (FI) - D136 (FV)	Wrong sequence for pFlgB	136	August 12
MP145.2						151
MP145.6						163
MP145.7						188
MP146.1	L130	[http://partsregistry.org/Part:BBa_J61002 J61002]		pFlhB-RFP D134 (FI) - D136 (FV)	Wrong sequence for pFlhB	298	August 12
MP146.2						142
MP146.7						170
MP146.8						143
MP147	L100	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		rbs TetR - ECFP D110 (BV) - D130 (BI)			August 13
MP148.1	L101	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		rbs TetR - GFP tripart D110 (BV) - D131 (BI) DO NOT WORK !!!!	MP148.2 ; MP148.3	109	August 13
MP148.2						123
MP148.3						123
MP149	L114	[http://partsregistry.org/Part:pSB2K3 pSB2K3]		AracpBAD - gfp tripart D126 (BV) - D131 (BI)			August 13
MP150	L120	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		tetR repressible promoter - ECFP D106 (BV) - D130 (BI)			August 13
MP151	L122	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		RBS-lasI - ECFP D107 (BV) - D130 (BI)			August 13
MP152	L123	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		RBS lasI - gfp tripart D107 (BV) - D131 (BI)			August 13
MP153.1	L126	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI)	MP153.1 ; MP153.3	100	August 13
MP153.2						131
MP153.3						113
MP154	L132	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		flhDC D142(FV) - D139(FI)			August 13
MP155	L133	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		OmpR* D142(FV) - D140(FI)	WT sequence (August 19)		August 13 ; August 19
MP156	L134	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		EnvZ* D142(FV) - D141(FI)	lacks about 300 bp in EnvZ* (August 19)		August 13 August 19
MP157	L138	[http://partsregistry.org/Part:pSB3K3 pSB3K3]		gfp generator (E0240) D137(FV) - D138(FI)			August 13
MP158	L139	[http://partsregistry.org/Part:pSB3K3 pSB3K3]		E0240 RBS+ D140 (PstI) - D144 (ES)	seems to have the plasmid without the insert and one with the insert		August 15
MP159	L141	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		flhD D142 (ES) - D145 (ES)	has not been sequenced		August 15
MP160	L142	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		flhDC+promotor D143 (ES) - D145 (ES)	does not have the insert		August 15
MP161	L150	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		pLas + rbs-tetR-gfp tripart D105 (SP) - D146 (XP)	incorrect clone -> to do again		August 19
MP162.1	L151.1	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]		rbs-lasR + double terminator D147 (ES) - D125 (EX)		151	August 19
MP162.2						172
MP162.3						149
MP163.1	[http://partsregistry.org/Part:BBa_B0032 B0032]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		RBS		80	August 20
MP163.2	[http://partsregistry.org/Part:BBa_B0032 B0032]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		RBS		79	August 20
MP164	[http://partsregistry.org/Part:BBa_E0422 E0422]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		RBS+ ECFP+ LVA+ term			August 20
MP165	[http://partsregistry.org/Part:BBa_E0430 E0430]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		RBS+ YFP+ LVA- term			August 20
MP166	[http://partsregistry.org/Part:BBa_E0432 E0432]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		RBS+ YFP LVA+ term BUT be careful , it doesn't work. See registry			August 20
MP167	[http://partsregistry.org/Part:BBa_E0420 E0420]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]		RBS+ ECFP LVA- term			August 20
MP168	[http://partsregistry.org/Part:BBa_I732078 I732078]	[http://partsregistry.org/Part:pSB1A3 pSB1A3]		RBS+ mRFP LVA+ term			August 20
MP169	[http://partsregistry.org/Part:BBa_J23100 J23100]-[http://partsregistry.org/Part:BBa_B0034 B0034 ]-[http://partsregistry.org/Part:BBa_C0079 C0079]-[http://partsregistry.org/Part:BBa_B0015 B0015]	[http://partsregistry.org/Part:J61002 J61002]		strong constitutive promoter-RBS-LasR-term			August 21
MP170	[http://partsregistry.org/Part:BBa_J23101 J23101]-[http://partsregistry.org/Part:BBa_B0034 B0034 ]-[http://partsregistry.org/Part:BBa_C0079 C0079]-[http://partsregistry.org/Part:BBa_B0015 B0015]	[http://partsregistry.org/Part:J61002 J61002]		strong constitutive promoter-RBS-LasR-term			August 21
MP171	L167	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	gfp generator(E0240) - pTet	gfp generator(E0240) - pTet			August 25
MP172	L166	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	RBS(B0032) - tetR	RBS(B0032) - tetR			August 25
MP173	L160	[http://partsregistry.org/Part:J61002 J61002 ]	pFlgA-YFP tripart LVA-	pFlgA-YFP tripart LVA- term			August 26
MP174	L161	[http://partsregistry.org/Part:J61002 J61002 ]	pFlgA-YFP tripart LVA+	pFlgA-YFP tripart LVA+ term			August 26
MP175	pFlhB	pCR4Blunt-TOPO	pFlhB	pFlhB-RBS (WT)			September 04
MP176	pFliA	pCR4Blunt-TOPO	pFliA	pFliA-RBS (WT) with conserved internal Pst1 restriction site			September 04
MP177	pFlhDC	pCR4Blunt-TOPO	pFlhDC	pFlhDC-RBS (WT)			September 04
MP178	pFliL	pCR4Blunt-TOPO	pFliL	pFliL-RBS (WT)			September 04
MP179	RBS WT-FlhDC	pCR4Blunt-TOPO	RBS(FlhDC) - FlhDC	RBS(FlhDC) - FlhDC with conserved internal Pst1 restriction site			September 04
MP180	FliA2	pCR4Blunt-TOPO	FliA	FliA mutated at EcoR1 and Pst1 internal site			September 04
MP181	FliA3	pCR4Blunt-TOPO	FliA3	FliA mutated at EcoR1 and Pst1 internal site			September 04
MP182	EnvZ*	pCR4Blunt-TOPO	EnvZ*	EnvZ* the good one but not mutated for the internal EcoR1 restriction site			September 04
MP183	OmpR WT	pCR4Blunt-TOPO	OmpR WT	OmpR WT not the mutated			September 04
MP184	pFlgB	pCR4Blunt-TOPO	pFlgB	pFlgB-RBS (WT)			September 04
MP185	OmpR WT		OmpR WT	OmpR WT not the mutated			September 04
MP200	J23101 + RBS (B0032) + tet R	[http://partsregistry.org/Part:J61002 J61002]		J23101 + RBS (B0032) + tet R			September 24
MP201	pFlgA-EnvZ*	[http://partsregistry.org/Part:J61002 J61002]	pFlgA-EnvZ*	pFlgA(RBS WT)-EnvZ*			September 24
MP202	L174; J23101-E0240	[http://partsregistry.org/Part:J61002 J61002]	Strong constitutive promoter-GFP generator J23101-E0240				September 26
MP207	pFliA	[http://partsregistry.org/Part:BBa_J61002 J61002]	pFliA	pFliA-RBS (WT)			October 04
MP208	pFlgA-EnvZ*	[http://partsregistry.org/Part:pSB3K3 pSB3K3]	pFlgA-EnvZ*	pFlgA(RBS WT)-EnvZ*			October 04
MP209	pFlgA-EnvZ*-[http://partsregistry.org/Part:BBa_I732078 I732078]	[http://partsregistry.org/Part:J61002 J61002]	pFlgA-EnvZ*-mRFP tripart	pFlgA(RBS WT)-EnvZ*-mRFP tripart			October 04
MP210	pFliL	[http://partsregistry.org/Part:pSB3K3 pSB3K3]	pFliL	pFliL(RBS WT)			October 10
MP211	pFlgA	[http://partsregistry.org/Part:pSB3K3 pSB3K3]	pFlgA	pFlgA(RBS WT)			October 10
MP212	pFlhB	[http://partsregistry.org/Part:pSB3K3 pSB3K3]	pFlhB	pFlhB(RBS WT)			October 10
MP213	pFlhB	[http://partsregistry.org/Part:BBa_J61002 J61002]	pFlhB(RBS WT)-mRFP	pFlhB(RBS WT)-mRFP			October 10
MP214	pFliL	[http://partsregistry.org/Part:BBa_J61002 J61002]	pFliL(RBS WT)-mRFP	pFliL(RBS WT)-mRFP			October 10
MP215		[http://partsregistry.org/Part:pSB4T5 pSB4T5]		pSB4T5 (low copy plasmid with pSC101 ORI / tetracycline resistance)			October 14
MP216	[http://partsregistry.org/Part:BBa_E0434 E0434]	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]		YFP tripart +AAV			October 15

Digestion Products

(Legend: Vector, Insert) Ø

Digestion Products
Number	Product Name	Matrix Name	Enzyme 1	Enzyme 2	Obs	Size Matrix (bp)	Size BB (bp)	Description	Product in use	[]ng/µL	Date
D100.1	[http://partsregistry.org/Part:BBa_B0034 B0034]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	34			10	July 26
D100.2	[http://partsregistry.org/Part:BBa_B0034 B0034]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	34			10	July 26
D101	[http://partsregistry.org/Part:BBa_B0034 B0034]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	XbaI	FV	2076	15			10	July 29
D102.1	[http://partsregistry.org/Part:BBa_B0034 B0034]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2077	14			10	July 26 August 15
D102.2	[http://partsregistry.org/Part:BBa_B0034 B0034]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2077	14			10	July 26 August 15
D103.1	[http://partsregistry.org/Part:BBa_J23101 J23101]	[http://partsregistry.org/Part:BBa_J61002 J61002]	SpeI	PstI	BV	2100	883			27	July 26
D103.2	[http://partsregistry.org/Part:BBa_J23101 J23101]	[http://partsregistry.org/Part:BBa_J61002 J61002]	SpeI	PstI	BV	2100	883			35	July 26
D104.1	[http://partsregistry.org/Part:BBa_J23109 J23109]	[http://partsregistry.org/Part:BBa_J61002 J61002]	SpeI	PstI	BV	2100	883			20	July 26
D104.2	[http://partsregistry.org/Part:BBa_J23109 J23109]	[http://partsregistry.org/Part:BBa_J61002 J61002]	SpeI	PstI	BV	2100	883			20	July 26
D105.1	[http://partsregistry.org/Part:BBa_R0079 R0079]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2222	14			5	July 26
D105.2	[http://partsregistry.org/Part:BBa_R0079 R0079]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2222	14			5	July 26
D106.1	[http://partsregistry.org/Part:BBa_R0040 R0040]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2119	14			8	July 26
D106.2	[http://partsregistry.org/Part:BBa_R0040 R0040]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2119	14			8	July 26
D107	[http://partsregistry.org/Part:BBa_S03154 S03154]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2750	14		August 23	5 12	July 26 July 23
D108.1	[http://partsregistry.org/Part:BBa_S03154 S03154]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	707			5	July 29
D108.2	[http://partsregistry.org/Part:BBa_S03154 S03154]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	707			5	July 29
D109	[http://partsregistry.org/Part:BBa_S03154 S03154]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	SpeI	FI	2056	708		July 26	10	July 26
D110	[http://partsregistry.org/Part:BBa_S03879 S03879]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2768	14		August 23	20 8 19	July 26 August 23 August 28
D111.1	[http://partsregistry.org/Part:BBa_S03879 S03879]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	725			20	July 26
D111.2	[http://partsregistry.org/Part:BBa_S03879 S03879]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	725			20	July 26
D112	[http://partsregistry.org/Part:BBa_S03879 S03879]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	SpeI	FI	2079	703			8	September 5
D113	[http://partsregistry.org/Part:BBa_C0079 C0079]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	EcoRI	SpeI	FI	4402	779			20	July 26
D114	[http://partsregistry.org/Part:BBa_C0079 C0079]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	XbaI	PstI	BI	4003	778			20	July 26
D115.1	[http://partsregistry.org/Part:BBa_C0179 C0179]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	SpeI	FI	4402	746			-	July 29
D115.2	[http://partsregistry.org/Part:BBa_C0179 C0179]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	SpeI	FI	4402	746			-	July 29
D116.1	[http://partsregistry.org/Part:BBa_C0179 C0179]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	4403	745			20	July 29
D116.1	[http://partsregistry.org/Part:BBa_C0179 C0179]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	4403	745			20	July 29
D117	[http://partsregistry.org/Part:BBa_E0030 E0030]	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	EcoRI	SpeI	FI	3166	746			5	July 29
D118	[http://partsregistry.org/Part:BBa_E0030 E0030]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	XbaI	PstI	BI	3167	745			20	July 26
D119	[http://partsregistry.org/Part:BBa_E0040 E0040]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	SpeI	FI	2056	743			10	July 26
D120	[http://partsregistry.org/Part:BBa_E0040 E0040]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	742			10	July 26
D121	[http://partsregistry.org/Part:BBa_E1010 E1010]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	EcoRI	SpeI	FI	4402	704			10	July 26
D122	[http://partsregistry.org/Part:BBa_E1010 E1010]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	4403	703			10	July 26
D123.1	[http://partsregistry.org/Part:BBa_J23100 J23100]	[http://partsregistry.org/Part:J61002 J61002]	SpeI	PstI	BV	2100	883			26	July 26
D123.2	[http://partsregistry.org/Part:BBa_J23100 J23100]	[http://partsregistry.org/Part:J61002 J61002]	SpeI	PstI	BV	2100	883			23	July 26
D124.1	[http://partsregistry.org/Part:BBa_J23107 J23107]	[http://partsregistry.org/Part:J61002 J61002]	SpeI	PstI	BV	2100	883			15	July 26
D124.2	[http://partsregistry.org/Part:BBa_J23107 J23107]	[http://partsregistry.org/Part:J61002 J61002]	SpeI	PstI	BV	2100	883			15	July 26
D125.1	[http://partsregistry.org/Part:BBa_B0015 B0015]	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	EcoRI	XbaI	FV	3303	15			26 10	July 26 August 23 August 28
D125.2	[http://partsregistry.org/Part:BBa_B0015 B0015]	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	EcoRI	XbaI	FV	3303	15			24 3687	July 26 August 23 August 28
D126.1	[http://partsregistry.org/Part:BBa_I0500 I0500]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	SpeI	PstI	BV	5621	14			10	July 26
D126.2	[http://partsregistry.org/Part:BBa_I0500 I0500]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	SpeI	PstI	BV	5621	14			10	July 26
D127.1	[http://partsregistry.org/Part:BBa_B0030 B0030]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	37			10	July 26
D127.2	[http://partsregistry.org/Part:BBa_B0030 B0030]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	37			10	July 26
D128.1	[http://partsregistry.org/Part:BBa_B0030 B0030]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	XbaI	FV	2079	15			10	July 29
D128.1	[http://partsregistry.org/Part:BBa_B0030 B0030]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	XbaI	FV	2079	15			10	July 29
D129.1	[http://partsregistry.org/Part:BBa_B0030 B0030]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2080	14			10	July 29
D129.2	[http://partsregistry.org/Part:BBa_B0030 B0030]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2080	14			10	July 29
D130.1	[http://partsregistry.org/Part:BBa_E0422 E0422]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	939			10	July 26
D130.2
D130.3
D131.1	[http://partsregistry.org/Part:BBa_E0840 E0840]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	900			8	July 26
D131.2
D131.3
D132	pFlgA	-	EcoRI	SpeI	FI	-	250			5	August 6
D133	pFlgB	-	EcoRI	SpeI	FI	-	250			5	August 6
D134	pFlhB	-	EcoRI	SpeI	FI	-	249			5	August 6
D135	pFlhDC	-	EcoRI	SpeI	FI	-		problem			August 6
D136	-	[http://partsregistry.org/Part:BBa_J61002 J61002]	EcoRI	SpeI	FV	2925	-				August 6 August 16
D137	pSB3K3	[http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3]	EcoRI	SpeI	FV	2727	-				August 6
D138	E0240	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	SpeI	FI	899	-				August 6
D139	flhDC	-	EcoRI	PstI	FI	-	972				August 6
D140	E040 rbs+	-	PstI	PstI	I	-					August 12
D141	flhD RBS -	-	EcoRI	SpeI	FI	-					August 12
D142	flhC RBS -	-	EcoRI	SpeI	FI	-					August 12
D143	flhDC + promoter	-	EcoRI	SpeI	FI	-					August 12
D144	pSB3K3	[http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3]	EcoRI	SpeI	FI	-					August 12
D145	pSB1A2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	SpeI	FI	-					August 12 August 15
D146	MP148	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	1609	rbs TetR - GFP tripart D110 (BV) - D131 (BI)			August 15
D147	MP153	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	SpeI	FI	2056	799	Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI)			August 15
D149	pfliL	-	EcoRI	SpeI	FI	-					August 14 August 16
D150	pfliL	-	XbaI	PstI	BI	-					August 14 August 16
D152	pSB3K3	[http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3]	XbaI	PstI	BI	-					August 14
D153	PCR 138 - gène flhDC	-	EcoRI	SpeI	FI	-					August 15
D154	PCR 140 - gène flhDC	-	EcoRI	SpeI	FI	-					August 15
D155	PCR 139 - pflhDC (URI)	-	EcoRI	SpeI	FI	-					August 15
D158	MP 155.1 - OmpR*	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	XbaI	PstI	BI	-					August 15
D159	MP 156.1 - EnvZ*	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	XbaI	PstI	BI	-					August 15
D161	MP 104 - pTet	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	SpeI	PstI	BV	-				39	August 16
D162	MP 114 - TetR	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	SpeI	FI	-				11	August 16
D163	MP 143 - gfp generator	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	XbaI	PstI	BI	-			August 23	14 11	August 16 August 23
D164	MP 101 - pJ23101	[http://partsregistry.org/Part:BBa_J61002 J61002]	SpeI	PstI	BV	-				3	August 16
D165	MP162.2	[http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3]	XbaI	PstI	BI	2057	798	rbs-lasR - double terminator D147 (FI) - D125 (FV)		27	August 18
D166	MP 165.2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	XbaI	FV	-	2942	RBS (B0034)+ YFP+ LVA- term B0015		59	August 26 September 3
D179	MP3.4 or MP118	[http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3]	SpeI	PstI	BV			B0015 (double terminator B0010-B0012)			August 20
D180	MP101.1	[http://partsregistry.org/Part:BBa_J61002 J61002]	SpeI	PstI	BV			promoter J23101			August 20
D181	MP104.2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	XbaI	FV			pTet (TetR repressible promoter)			August 20
D182	MP114.1	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	XbaI	PstI	BI			TetR			August 20
D183	MP119.3	-	XbaI	PstI	BI			pBad promoter			August 20
D184	MP143.1	[http://partsregistry.org/Part:BBa_pSB2K3 pSB2K3]	EcoRI	SpeI	FI			gfp generator			August 18
D185	MP163.1	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	SpeI	PstI	BV			B0032 RBS			August 18
D187	MP168.1	[http://partsregistry.org/Part:BBa_pSB1A3 pSB1A3]	EcoRI	XbaI	FV			RBS (B0034)+ mRFP LVA+ term B0015		43	September 3
D188	PCR 138	-	XbaI	PstI	Insert	-	767	OmpR*			August 26
D189	MP 101.2	[http://partsregistry.org/Part:BBa_J61002 J61002]	XbaI	PstI	Vector	-	2073	pSB1A2			August 26
D191	MP114.2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	SpeI	PstI	BV		2724	tetR		22	August 29
D192	MP144.2	[http://partsregistry.org/Part:BBa_J61002 J61002]	EcoRI	XbaI	FV		2267	FlgA		26	August 29
D193	MP171	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	SpeI	FI		961	gfp generator(E0240) - pTet		9,5	August 29
D194	MP172.2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	SpeI	FI		704	RBS(B0032) - tetR		13	August 29
D195	MP172.2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	XbaI	PstI	BI		703	RBS(B0032) - tetR		11	August 29
D196	MP104.2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	SpeI	PstI	BV		2119	pTet		45	September 3
D197	MP157.2	[http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3]	XbaI	PstI	BI		898	gfp generator(E0240)		12	September 3
D198	MP164.2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	XbaI	FV		2981	RBS (B0034)+ ECFP+ LVA+ term B0015		36	September 3
D199	MP167.1	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	XbaI	FV		2942	RBS (B0034)+ ECFP LVA- term (B0015)		61	September 3
D200	MP171.2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	XbaI	PstI	BI		960	gfp generator(E0240) - pTet		43	September 3
D201	MP114.2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	XbaI	FV		2724	tetR		13	August 29
D202	MP101.1	[http://partsregistry.org/Part:BBa_J61002 J61002]	EcoRI	nothing			2984			25	September 8
D203	MP101.2	[http://partsregistry.org/Part:BBa_J61002 J61002]	BspHI	nothing			1870 1008 105				September 11
D204	MP144	[http://partsregistry.org/Part:BBa_J61002 J61002]	SpeI	PstI							September 22
D205	MP101	[http://partsregistry.org/Part:BBa_J61002 J61002]	XbaI	PstI							September 22
D206	MP179		XbaI	PstI							September 22
D207	MP182.3		XbaI	PstI							September
D208	MP175		EcoRI	SpeI							September
D209	MP144	[http://partsregistry.org/Part:BBa_J61002 J61002]	EcoRI	SpeI							September
D210	MP178		EcoRI	SpeI							September

Strains (glycerol stock)

Strains glycerol stock
Number	Name	Biobricks	Description	Verified clone	Date
S103	[http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3]		AraC pBAD		July 23
S106	[http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]		ECFP (RBS+LVA+ Double Term)		July 23
S107	[http://partsregistry.org/Part:BBa_B0034 B0034] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strongest RBS (Efficiency = 1)		July 23
S108	[http://partsregistry.org/Part:BBa_R0079 R0079] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		promoter pLas (lasr & PAI regulated)		July 23
S109	[http://partsregistry.org/Part:BBa_J23101 J23101] in [http://partsregistry.org/Part:BBa_J61002 J61002 ]		Strongest Constitutive promoter		July 23
S110	[http://partsregistry.org/Part:BBa_J23109 J23109] in [http://partsregistry.org/Part:BBa_J61002 J61002 ]		Weak Constitutive promoter		July 23
S111	[http://partsregistry.org/Part:BBa_R0040 R0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		tetR repressible promoter		July 23
S112	[http://partsregistry.org/Part:BBa_S03154 S03154] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		B0034(rbs) ->LasI		July 23
S113	[http://partsregistry.org/Part:BBa_S03879 S03879] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		B0034(rbs) -> TetR		July 23
S114	[http://partsregistry.org/Part:BBa_C0079 C0079] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]		lasR activator with LVA Tag		July 23
S115	[http://partsregistry.org/Part:BBa_C0179 C0179] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		lasR activator		July 23
S116	[http://partsregistry.org/Part:BBa_E0030 E0030] in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]		YFP		July 23
S117	[http://partsregistry.org/Part:BBa_E0040 E0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		GFP		July 23
S118	[http://partsregistry.org/Part:BBa_E1010 E1010] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]		mRFP		July 23
S119	OmpR M57I				July 23
S120	EnvZ I281S				July 23
S121	[http://partsregistry.org/Part:BBa_C0040 C0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		tetR repressor		July 23
S122	[http://partsregistry.org/Part:pSB1A3 pSB1A3]				July 23
S123	L102 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strong rbs - YFP D129 (BV) - D118 (BI)		Aug 1
S124	L103 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strong rbs - mRFP D129 (BV) - D122 (BI)		Aug 1
S125	L104 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strong rbs - lasR activator D129 (BV) - D114 (BI)		Aug 1
S126	L105 in [http://partsregistry.org/Part:J61002 J61002]		Strong promoter - ECFP D123 (BV) - D130 (BI)		Aug 1
S127	L106 in [http://partsregistry.org/Part:J61002 J61002]		Strong promoter - gfp Tripart D123 (BV) - D131 (BI)		Aug 1
S128	L107 in [http://partsregistry.org/Part:J61002 J61002]		Strongest promoter - ECFP D103 (BV) - D130 (BI)		Aug 1
S129	L108 in [http://partsregistry.org/Part:J61002 J61002]		Strong promoter - GFP tripart D103 (BV) - D131 (BI)		Aug 1
S130	L110 in [http://partsregistry.org/Part:J61002 J61002]		Medium promoter - gfp tripart D124 (BV) - D131 (BI)		Aug 1
S131	L111 in [http://partsregistry.org/Part:J61002 J61002]		Weak promoter - ECFP D104 (BV) - D130 (BI)		Aug 1
S132	L112 in [http://partsregistry.org/Part:J61002 J61002]		Weak promoter - gfp Tripart D104 (BV) - D131 (BI)		Aug 1
S133	L115 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		pLas - ECFP D105 (BV) - D130 (BI)		Aug 1
S134	L116 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		pLas - gfp Tripart D105 (BV) - D131 (BI)		Aug 1
S135	L117 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]		yfp - Double Terminator D125 (FV) - D117 (FI)		Aug 1
S136	L118 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]		rfp - Double Terminator D125 (FV) - D121 (FI)		Aug 1
S137	L119 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]		LasR activator + LVA - Double terminator D125 (FV) - D113 (FI)		Aug 1
S138	L121 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		pTet - gfp Tripart D106 (BV) - D131 (BI)		Aug 1
S139	L124 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strongest rbs - mRFP D102 (BV) - D122 (BI)		Aug 1
S140	L125 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strongest rbs - yfp D102 (BV) - D118 (BI)		Aug 1
S141	MG1655				Aug 7
S142	[http://partsregistry.org/Part:J61002 J61002]				Aug 7
S143	L128 in [http://partsregistry.org/Part:J61002 J61002]		pFlgA-RFP D132 (FI) - D136 (FV)	MP144.3 ; MP144.4	August 12
S144	L129 in [http://partsregistry.org/Part:J61002 J61002]		pFlgB-RFP D133 (FI) - D136 (FV)	Wrong sequence for pFlgB	August 12
S145	L130 in [http://partsregistry.org/Part:J61002 J61002]		pFlhB-RFP D134 (FI) - D136 (FV)	Wrong sequence for pFlhB	August 12
S146	L100 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		rbs TetR - ECFP D110 (BV) - D130 (BI)		August 13
S147	L101 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		rbs TetR - GFP tripart D110 (BV) - D131 (BI)		August 13
S148	L114 in [http://partsregistry.org/Part:pSB2K3 pSB2K3]		AracpBAD - gfp tripart D126 (BV) - D131 (BI)		August 13
S149	L120 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		tetR repressible promoter - ECFP D106 (BV) - D130 (BI)		August 13
S150	L122 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		RBS-lasI - ECFP D107 (BV) - D130 (BI)		August 13
S151	L123 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		RBS lasI - gfp tripart D107 (BV) - D131 (BI)		August 13
S152	L126 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI)		August 13
S153	L132 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		flhDC D142(FV) - D139(FI)		August 13
S154	L133 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		OmpR* D142(FV) - D140(FI)		August 13
S154	L133.1 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		OmpR* D142(FV) - D140(FI)	WT sequence	August 19
S155	L134 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		EnvZ* D142(FV) - D141(FI)		August 13
S155	L134.2 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		EnvZ* D142(FV) - D141(FI)	lacks about 300 bp in EnvZ*	August 19
S156	L138 in [http://partsregistry.org/Part:pSB3K3 pSB3K3]		gfp generator (E0240) D137(FV) - D138(FI)		August 13
S157	[http://partsregistry.org/Part:BBa_E0240 E0240] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		GFP generator		August 15
S158	[http://partsregistry.org/Part:pSB3K3 pSB3K3]		plasmid		August 15
S159.1	L139.1 in [http://partsregistry.org/Part:pSB3K3 pSB3K3]		E0240 RBS+ D140 (PstI) - D144 (ES)	seems to have the plasmid without the insert and one with the insert	August 15
S159.2	L139.1 in [http://partsregistry.org/Part:pSB3K3 pSB3K3]		E0240 RBS+ D140 (PstI) - D144 (ES)	incorrect clone	August 15
S160.1	L141.1 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		flhD D142 (ES) - D145 (ES)	has not been sequenced	August 15
S160.2				has not been sequenced
S160.3				has not been sequenced
S161.1	L142.7 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		flhDC+promotor D143 (ES) - D145 (ES)	does not seem to have the insert	August 15
S161.2	L142.7 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		flhDC+promotor D143 (ES) - D145 (ES)	incorrect clone	August 15
S162	L151.1 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]		rbs-lasR + double terminator D147 (ES) - D125 (EX)		August 18
S163	[http://partsregistry.org/Part:BBa_B0032 B0032] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		medium rbs (efficiency = 0,3)		August 20
S164	[http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		strongest rbs ECFP LVA+ double terminator		August 20
S165	[http://partsregistry.org/Part:BBa_E0430 E0430] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		strongest rbs YFP LVA- double terminator		August 20
S166	[http://partsregistry.org/Part:BBa_E0432 E0432] in [http://partsregistry.org/Part:pSB1A2 pSB1A2 ]		strongest rbs YFP LVA+ double terminator		August 20
S167	[http://partsregistry.org/Part:BBa_E0420 E0420] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		strongest rbs ECFP LVA- double terminator		August 20
S168	[http://partsregistry.org/Part:BBa_I732078 I732078] in [http://partsregistry.org/Part:pSB1A3 pSB1A3]		strongest rbs mRFP LVA+ double terminator		August 20
S169	[http://partsregistry.org/Part:BBa_J23100 J23100]-[http://partsregistry.org/Part:BBa_B0034 B0034 ]-[http://partsregistry.org/Part:BBa_C0079 C0079]-[http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:J61002 J61002]		strong constitutive promoter-RBS-LasR-term		August 21
S170	[http://partsregistry.org/Part:BBa_J23101 J23101]-[http://partsregistry.org/Part:BBa_B0034 B0034 ]-[http://partsregistry.org/Part:BBa_C0079 C0079]-[http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:J61002 J61002]		strong constitutive promoter-RBS-LasR-term		August 21
S171	L121 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		pTet - gfp Tripart D106 (BV) - D131 (BI)	Same as S138 but in chemically labmade competent MG1655 bacteria. For study of doubling time of population	Aug 22
S172	L167 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		gfp generator(E0240) - pTet		Aug 25
S173	L166 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		RBS(B0032) - tetR		Aug 25
S174	pFlgA-[http://partsregistry.org/Part:BBa_E0430 E0430] in [http://partsregistry.org/Part:J61002 J61002]		pFlgA-YFP tripart LVA-	Fluorescence OK	August 26
S175	pFlgA-[http://partsregistry.org/Part:BBa_E0432 E0432] in [http://partsregistry.org/Part:J61002 J61002]		pFlgA-YFP tripart LVA+		August 26
S178	pFlhB in pCR4Blunt-TOPO		pFlhB		September 4
S179	pFliA in pCR4Blunt-TOPO		pFliA with conserved Pst1 restriction site		September 4
S180	pFlhDC in pCR4Blunt-TOPO		pFlhDC		September 4
S181	pFliL in pCR4Blunt-TOPO		pFliL		September 4
S182	RBS (WT)-FlhDC in pCR4Blunt-TOPO		RBS (WT)-FlhDC with conserved Pst1 restriction site		September 4
S183	FiA2 in pCR4Blunt-TOPO		FliA2 (mutated FliA at internal EcoR1 and Pst1 restriction site)		September 4
S184	FiA3 in pCR4Blunt-TOPO		FliA3 (mutated FliA at internal EcoR1 and Pst1 restriction site)		September 4
S185	EnvZ* in pCR4Blunt-TOPO		EnvZ* (non regulated EnvZ with conserved EcoR1 restriction site)		September 4
S186	OmpR* in pCR4Blunt-TOPO		OmpR* (Not mutated but WT gene)		September 4
S187	pFlgB in pCR4Blunt-TOPO		pFlgB		September 4
S188	OmpR* in pCR4Blunt-TOPO		OmpR* (Not mutated but WT gene again)		September 4
S200	L179 in [http://partsregistry.org/Part:J61002 J61002]		J23101 + RBS (B0032) + tet R		September 24
S201.1	pFlgA(+RBS)-envZ* in [http://partsregistry.org/Part:J61002 J61002]		pFlgA(+RBS)-envZ*		September 24
S201.2
S201.3
S201.4
S201.6
S207	pFliA in [http://partsregistry.org/Part:BBa_J61002 J61002]		pFliA-RBS (WT)		October 04
S208	pFlgA-EnvZ* in [http://partsregistry.org/Part:pSB3K3 pSB3K3]		pFlgA(RBS WT)-EnvZ*		October 04
S209	pFlgA-EnvZ*-[http://partsregistry.org/Part:BBa_I732078 I732078] in [http://partsregistry.org/Part:J61002 J61002]		pFlgA(RBS WT)-EnvZ*-mRFP tripart		October 04
S210	pFliL in [http://partsregistry.org/Part:pSB3K3 pSB3K3]		pFliL(RBS WT)		October 10
S211	pFlgA in [http://partsregistry.org/Part:pSB3K3 pSB3K3]		pFlgA(RBS WT)		October 10
S212	pFlhB in [http://partsregistry.org/Part:pSB3K3 pSB3K3]		pFlhB(RBS WT)		October 10
S213	pFlhB in [http://partsregistry.org/Part:J61002 J61002]		pFlhB(RBS WT)- mRFP		October 10
S214	pFliL in [http://partsregistry.org/Part:J61002 J61002]		pFliL(RBS WT)- mRFP		October 10
S215	[http://partsregistry.org/Part:pSB4T5 pSB4T5]		plasmid		October 14