Competition tests

Piotr

1. Plasmid isolation from previous day's cultures.
2. Digest with SacI and BamHI.
3. Electrophoresis.

Conclusion: cells with interracting protein survive competition!

MutD5 testing

Piotr

Inoculation of MutD5 into medium with tetracycline.

Optimisation of primers for preparation of parts

Michał K.

1. PCR on pACYC177+OmpA_omega_deltaA_alpha plasmid using AL_BNXNE and AP+XhoI+SpeI primers (elongation length 45s).
2. PCR on pACYC177+OmpA_omega_deltaA_alpha plasmid using LinLSXNE and AlfaPSpe primers (elongation length 45s). Fig. 2 and Fig. 3.
3. PCR on pUC19 plasmid using OmegaL_link and OmegaP_EPB primers (elongation length 30 s). Fig. 3 and Fig. 4.

Each reaction was carried out with 15 and 20 cycles and in temperature gradient from 55 to 75 °C.