Change of the reporter from pZC with B-galactosidaze to GFP or RFP

Piotr, Weronika

1. There were blue and white colonies but no red or green in UV
2. Inoculation of liquid LB medium + Amp with white colonies
3. Inoculation of 10ml LB with E.coli TOP10 carrying pZC
4. Inoculation of E.coli TOP10 carrying nazwa (Gfp genetrator), nazwa (RFP generator)

Cloning alpha-A and omega-A fusions on pKS

Michał L., Ewa, Marcin

Inoculation of liquid LB medium with 6 transformant colonies.

Preparation of constructs with OmpA protein fusions

Paweł

Inoculation of E. coli with pET15b plasmid on LB + ampicillin.