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Waterloo/20 June 2008
From 2008.igem.org
08/06/20 Friday
To do:
[x] Make competent cells
[x] Mini prep PluxR-gfp"
[x] Diagnostic digest of mini preps (and run on gel) - 1 + 1.5 hr
- I think all transformants worked, but the ladder ran a bit weird, so we might have to check it again to make sure.
[x] Repeat PluxRgfp" (1-8) with gfp`` positive control
[x] Prepare glycerol stock of registry parts
[x] Patch streak inoculate of Registry transformations - 1 hr? - will be taken out of 37 and put into 4 degree on saturday.
[x] determined which registry parts we have from 2007, and box locations
[x]diagnostic digest and gel of luxI and PlaclasI
- inserts observed, but sizes are really off.
- luxI`` (#1-4) wrong insert.
