Waterloo/24 June 2008

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08/06/24 Tuesday

[x] streak/patch/innoculate 2007 and 2008 registry parts (13:00 - 14:00)

[x] re-transform one sample from yesterday and PocI (13:00)
- No growth

[x] diagnostic digest of PluxRgfp" (1-8) with gfp`` positive control (repeat from 20/6), and with sketchy DNA from 23/6 digest (TT, CI, PtetR, PoCI, PlacoCI, Pcost) (~1.5 hr)
[x] gel (1.5hr)

[x] analyze 07registry gel from yeserday
- Bad ladder, some samples ran ok for extraction, but needs to repeat other ones with better control

[x] prepare more LB tubes and LB agar
[x] make frozen stocks (20uL) of :
- PocI
and for the following also prepare eluted DNA.
- PcI (R0052-2-9G)
- PrecA (J22106-3-12F)
- TT (B0014-1-1A)
innoculate after confirming with dignostic gel