Waterloo/26 June 2008

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08/06/26 Thursday

  • Should have all the registration parts in by today !!*

[x] Repeat Gel of 2007 Registry

[x] streak
- cc on LB
- Placgfp Transformation cont

[x] take plates out of 37, pick colonies if transformation worked and innoculate to miniprep on Friday

PCR testing of Moffat Lab Taq, Charles Lab Taq, and Charles Lab Pfu

PCR reactions carried out with:
-1:100 dilution Moffat Taq
- undiluted Charles Taq (from stock in freezer)
- 1:10 charles Taq
- 1:100 Charles Taq
- The same dilutions with Charles Pfu.
- Also tested Pfu with hermopol buffer and fermentas PCR master mix
- Used gfp' mini prep as template

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