"
Waterloo/9 July 2008
From 2008.igem.org
08/07/09 Wed
[x] Diagnostic digest and gel of TTr (2 and 5, uncut and single cut) + Promoters (single cut + uncut + control)
[x] full digest of rfp' and tt and gel and gel extraction
[x] full digest of cI and rbs and gel and gel extraction
[x] gel of diagnostic and full digestion only half of diagnostic digest run on gel
- diagnostic - Plac1 (1-5), Plac A(1-5), PlacIq(1-2), PlacOCI (1-2), Plac (control), RBS and uncuts run.
- full - RBS, RBS, CI, CI, RFP', TT, TT, TT
- rest of the digest had loading dye added and left in p3
[x] cut out gel for extraction tomorrow (tt, cI, rfp', rbs)
[] make competent cells (cont...)
- will continue tomorrow
[x] update Parts Inventory list - confirm all parts + store in same box
