Waterloo/16 July 2008

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08/07/16 Wed

[] colony PCR of CI' (51) and CI' (52)
[] inoculation
[] test Pfu with new buffer at the same time
- no growth in ligation plates of c0052 and c0051
- no PCR. growth observed in rfp

[x] make glycerol stock for R0010, R0040 B0034, C0051, C0052

[x] miniprep of innoculations

[x] diagnostic gel (run with digest of PtetR and PlacOCI, and inoculations of colonies [C0052, R0010, R0040 B0034, C0051, C0052])

[x] gel extraction and purification

[x] If transformations did not work, ligation

[x] p/s/i of rfp

[x] inoculate chloroamphenical (cm)

[x] observe plasOCIrfp
- we see red