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Waterloo/17 July 2008
From 2008.igem.org
08/07/17 Thurs
[x] miniprep of transformants inoculations (rfp)
[x] diagnostic digest of transformants and c0051, c0052
[x] gel of diagnostic digest
[x] glycerol stocks of PFU innoculations
[x] p/s/i c0052'
[x] if gel worked, full digestion of Plac, POCI, Pconst (two of each), rfp, gfp
[x] gel of full digestion
[x] cut out bands of full digestion
[x] Inoculate Placgfp into liquid culture to test that way under UV
[x] inoculate c0052-1(B50), c0052-2(b51), c0051
[] dna extraction and ligation
[x] Run PCR and gel to test Pfu in Pfu specific buffer
- PCR didn't seem to work.
