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Beijing Normal/10 October 2008
From 2008.igem.org
pPcbC was inserted onto the PZE12 vector with YFP. The result will be come out tomorrow. And we generalized some key words for successful plasmids construction. Firstly, enzyme digestion time should be long enough, especially when double digestion was performed. Generally speaking, we performed it for 5h when some enzyme's activity is relatively low in the given buffer.
