Team:Caltech/Protocols/H2O2 Production Assay

An overnight culture of JI377 transformed with luxR+GFP or luxR (negative control) on pSB1A2 in SOC +Amp was back diluted 1:100 into 5 ml SOC +Amp +IPTG in triplicate. The cultures were grown to an OD600 of ~0.8, at which point the supernatant was assayed for hydrogen peroxide approximately every 30 minutes. 1hr after reaching an OD600 of ~0.8, the cultures were induced with 10 nM AHL.