Team:Tokyo Tech/Project

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Project

Code name Rising Sun

E.coli UKIYO-E

This project aims to make UKIYO-E by E.coli. UKIYO-E is a genre of Japanese woodblock prints.



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Contents

We want to make a Coli Touch!

We try to construct a bacterial `touch panel’ which is colored by the pressure. We name it Coli. touch.

  • why pressure ?
In iGEM, genetic circuits responded by various inputs -- for example, heat, small molecule, and light -- have been constructed. However, a pressure-responsive genetic circuit had not been constructed yet. Therefore, we constructed a pressure responsive circuit using a pressure-inducible promoter.


  • Pressure inducible promoter - Plac
The affinity of LacI for the lac operator is known to decrease due to a tetramer to dimer transition of LacI

Strategy

Project Details

Part 2

The Experiments

Part 3

Results