Cloning of protein Z DNA to pET15b+Omp-alpha plasmid

Antoni

1. Isolation of pET15b+Z-alpha plasmids.
2. Control digest of isolated plasmids with NdeI and NotI (Orange buffer).
3. Gel electrophoresis. Choice of proper clone.

Cloning of protein A DNA to pET15b+Omp-alpha plasmid

Michał K.

1. Colony PCR on colonies from plates with transformation pEt15b+A_alpha.
   Primers used: AP+NotI and AL+SacI.
2. Gel electrophoresis.
3. Confirmed transformant colonies inoculated to liquid LB with ampicillin.