Preparation of alpha-A and omega-A fusions

Michał L., Ewa, Marcin

Still no success. We need to run gradient PCR to find optimal reaction conditions.

Cloning of protein Z DNA to pET15b-OmpA-alpha in place of OmpA

Paweł

1. Control digest of isolated plasmids with NdeI and NotI. Two positives obtained (proper band: ~200 bp visible).
2. One of positive plasmids transformed into TOP10 and plated on LB+amp, overnight, for further isolation of pET15b+Z+omega vector.

Preparation of constructs with OmpA protein fusions

Michał K.

1. PCR on isolated plasmids with OmpaL_N and OmpaP_link primers.
2. Gel electrophoresis (Fig. 1, no proper clones found).