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Team:Warsaw/Calendar-Main/27 August 2008
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Cloning of protein A DNA to
pET15b+OmpA-alpha
plasmid
Michał K.
Digest
of
pGeneart+A
and
pET15b+Omp-alpha
with NdeI and NotI (Orange buffer), pET15b was also
dephosphorylated
.
Gel electrophoresis of digested plasmids and
gel-out
of proper bands (400 bp for A lane and 6200 bp for pET15b lane).
Ligation
of
pET15b+alpha and A
.
Transformation of
E. coli
TOP10
.
Transformants plating on LB + ampicillin.
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