Team:Warsaw/Calendar-Main/29 August 2008

From 2008.igem.org

Revision as of 21:02, 19 October 2008 by Smaegol (Talk | contribs)

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Cloning of protein A DNA to pET15b+Omp-alpha plasmid

Michał K.

  1. Isolation of pET15b+A-alpha plasmids.
  2. Control digest of isolated plasmids with NdeI and NotI (Orange buffer).
  3. Gel elctrophoresis. Chioce of proper clone.

Protein purification: A-alpha

Piotr

Single transformations of Rosetta with plasmids pET15b+A-alpha. Plating on LB with chloramphenicol and ampicillin.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/29_August_2008"