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Waterloo/14 August 2008
From 2008.igem.org
8/08/14 Thurs
[x] send gene 6 for sequencing
[] p/s/i of c0052', c0051', T7 gene 6 + RBS
[x] miniprep of inoculatants from wed
[x] Gel of overnight PCR of phbA and phbB
[] if PCR didn't work - keep testing PCR conditions
- PCR worked
[] pjet gene 5 ligation
[] pjet gene 5 transformation
[x] check gel of pjet extractions gene 5
- note: PCR worked, but no DNA was seen from minprep samples
[x] PCR - PHB testing again.
[x] colony PCR condition testing
[x] patch colonies from transformation
