|
A simple synthesis-degradation model is assumed for the modelling of the expression of a protein under the control of a constitutive promoter, with the same model assumed for all four promoter-RBS constructs. The synthesis-degradation model assumes a steady state level of mRNA.
In this case, [protein] represents the concentration of GFP, k1 represents the rate of sythesis and d1 represents the degradation rate.
We can easily simulate this synthesis-degradation model using matlab:
ODE
Simulation File
We can also solve this ODE analytically. Consider the steady-state behaviour of [protein].
This relationship can be seen in the parameter scan graphs on the right.
From the wetlab experiments it is likely that we will obtain steady-state data for each of the four promoter-RBS constructs. If we assume the same rate of degradation of GFP in each case, we can have some measure of the relative rate of transcription through each promoter which will help us with the selection of the most appropriate promoter to use for Phase 2. In order to obtain an absolute measure of transcription (as opposed to a relative measure of transcriptional strength) we require constitutive expression in terms of molecules per cell (as opposed to fluorescence in arbitrary units).
Note from the parameter scan graphs:
- In the case where k1 = 0, no GFP is sythesised.
- In the case where d1 = 0, the concentration of protein does not reach a steady state.
|
 Constitutive expression of antibiotic resistance (AB) and GFP. GFP brick is part E0040, GFPmut3b. Terminator is part B0015, the double-stop. |
"
