Team:UCSF/Willis Wong Notebook

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Willis Wong Notebook

Overall Objective: 

   Devise an array of inputs that can be linked to regulation of silencing.

Milestones: 

  1. Define minimal fragment for light inducible dimerization.
  2. Define the configuration of dimerization/regulatory domains that will yield desired function. (Silencing / 
     Unsilencing)


Work Done: 

         Week 1-2: Design AB and BD parts for combinatorial cloning (includes PhyB and Pif3 parts).
         Week 3-4: Ligate AB + BD parts into 315 vectors.
         Week 4-5: Subclone Pif3 constructs into 303 vectors for intregration into a yeast reporter strain [B3(H)].
         Week 7-9: Transform the PhyB constructs into the yeast strains that were integrated with Pif3.
         Week 10-11: In the dark, added PCB, pulsed with respective light, ran FACs.
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