Team:Chiba/Calendar-Home/23 August 2008

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Contents

1 Laboratory work

Laboratory work

Team:Input

no work

Team:Communication

(21/8)--->Mini prep

BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]

--->Digestion test

BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]

Sample No	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21
BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]	3	-	-	-	-	-	-	3	-	-	-	-	-	-	3	-	-	-	-	-	-
BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]	-	3	-	-	-	-	-	-	3	-	-	-	-	-	-	3	-	-	-	-	-
BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]	-	-	3	-	-	-	-	-	-	3	-	-	-	-	-	-	3	-	-	-	-
BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]	-	-	-	3	-	-	-	-	-	-	3	-	-	-	-	-	-	3	-	-	-
BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]	-	-	-	-	3	-	-	-	-	-	-	3	-	-	-	-	-	-	3	-	-
BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]	-	-	-	-	-	3	-	-	-	-	-	-	3	-	-	-	-	-	-	3	-
BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]	-	-	-	-	-	-	1	-	-	-	-	-	-	3	-	-	-	-	-	-	3
EcoRⅠ	-	-	-	-	-	-	-	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1
PstⅠ	-	-	-	-	-	-	-	-	-	-	-	-	-	-	0.1	0.1	0.1	0.1	0.1	0.1	0.1
Buffer EcoRⅠ	-	-	-	-	-	-	-	0.9	0.9	0.9	0.9	0.9	0.9	0.9	0.8	0.8	0.8	0.8	0.8	0.8	0.8
BSA	-	-	-	-	-	-	-	1	1	1	1	1	1	1	1	1	1	1	1	1	1
dH2O	-	-	-	-	-	-	-	5	5	5	5	5	5	5	5	5	5	5	5	5	5
TOTAL	3	3	3	3	3	3	1	10	10	10	10	10	10	10	10	10	10	10	10	10	10

Sample No	1~6	7
Sample DNA	3	1
Loading Dye	1	1
dH2O	2	4
TOTAL	6	6

From left; Sanple No. 1~7

BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]

Sample No	8~21
Sample DNA	10
Loading Dye	2
TOTAL	12

From left;

(Single Digestion) Sanple No. 8~14

BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154] -> OK
BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154] -> OK
BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] -> OK
BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] -> OK
BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] -> OK
BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] -> OK
BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] -> OK

(Double Digestion) Sample No. 15~21

BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154] -> OK
BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154] -> OK
BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] -> OK
BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] -> OK
BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] -> OK
BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] -> OK
BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] -> OK?

Digestion test

BBa_J04500(2007:old-sample)[http://partsregistry.org/Part:BBa_J04500]

Sample No	1	2	3
Sample DNA	3	3	3
EcoRⅠ	-	0.1	0.1
SpeⅠ	-	-	0.1
EcoRⅠ Buffer	-	0.9	0.8
BSA	-	1	1
dH2O	-	5	5
TOTAL	3	10	10

--->Gel Check

Sample No	1	2	3
Sample DNA	3	10	10
Loading Dye	1	2	2
dH2O	2	-	-
TOTAL	6	12	12

From left;

BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] -> OK
BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Single Digestion -> OK
BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Double Digestion -> Bad

Team:Output

no work

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