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Team:Chiba/Calendar-Home/8 September 2008
From 2008.igem.org
7 September 2008 <|> 9 September 2008
Contents |
Laboratory work
Team:Input
no work
Team:Communication
-->(7/9)
- --->Zymo Clean
- ->15μL?
9 September, 2008
-->(6/9)PCR of I9030
--->Gel Check
--->Gel Extract
--->Zymo Clean
Team:Output
- [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)①
- [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](pLac)⑤
| Sample No. | ① | ⑤ | |
| DNA(digested sample) | 7 | 6 | |
| SAP/td> | 2 | 2 | |
| SAP Buffer | 3 | 3 | |
| TOTAL | 30 | 30 | |
| TOTAL | 30 | 30 |
-->37℃ 1hour
-->65℃ 30min
-->21μl 6μl
-->Gel check
| Sample No. | each |
| DNA(digested sample) | 1 |
| Loading Dye | 1 |
| dH2O | 4 |
| TOTAL | 6 |
- vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)① 75ng/μl
- insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)② 33.6ng/μl
- insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)③ 33.6ng/μl
- insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)④ 38.4ng/μl
- vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](pLac)⑤ 100ng/μl
- insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](RBS+rLuc)⑥ 33.6ng/μl
| Sample No. | ①&② | ①&③ | ①&④ | ① | ⑤&⑥ | ⑤ |
| DNA(vector) | 3 | 3 | 3 | 3 | 5 | 2.1 |
| DNA(insert) | 3 | 3 | 3 | - | 3.1 | - |
| Ligase | 1 | 1 | 1 | 1 | 1 | 1 |
| Ligase Buffer(x5) | 2 | 2 | 2 | 2 | 2 | 2 |
| dH2O | 1 | 1 | 1 | 4 | 8.9 | 4 |
| TOTAL | 10 | 10 | 10 | 10 | 20 | 10 |
