Team:Chiba/Calendar-Home/16 September 2008

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15 September 2008 <|> 17 September 2008

Laboratory work

Team:Communication

(15/9)->

  1. strain
    Senders:JW1908
    Receiver:JW1908
  2. temparature
    37℃ and 30℃


Team:Output


Digestion test

  • [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)(x2)
  • [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)(x5)
  • [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)(x2)
  • [http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)
  • [http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)
  • [http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)

</tr>

Sample No. each
DNA 3
EcoRⅠ 0.083
PstⅠ 0.083
BSA(x10) 1
Buffer3(x10) 1
dH2O 5
TOTAL 10

-->37℃ 1hour

Gel check

Sample No. each
DNA 10
Loading Dye 2
TOTAL 12

Transformation

  • [http://partsregistry.org/Part:BBa_J37019 BBa_J37019](LuxI+RBS+LuxR)->no colony
  • [http://partsregistry.org/Part:BBa_J37033 BBa_J37033](RBS+LuxR)
  • [http://partsregistry.org/Part:BBa_J37020 BBa_J37020](LuxI+LuxR+pLux+GFP)
  • [http://partsregistry.org/Part:BBa_J37032 BBa_J37032](pLux+GFP)

PCR

  • Venus YFP(ptet+LuxR+YFP)
Sample No. YFP
DNA 1
Venus Fwd 5
Rev (VR) 5
Thermo pol Buffer 10
dNTP 10
Vent 1
dH2O 68
TOTAL 100
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