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Team:Chiba/Calendar-Home/17 September 2008
From 2008.igem.org
16 September 2008 <|> 18 September 2008
Laboratory work
Team:Communication
- time-delay test
- conditions
- strain
Senders:JW1908
Receiver:JW1908
- temparature
37℃ and 30℃
- time-delay test
- conditions
- strain
Senders:XL10GOLD
Receiver:JW1908
- temparature
37℃ and 30℃
Team:Output
- BBa_I13500(RBS+GFP)
- BBa_J37019(Luxpr+LuxR)-->no colony
MINI Prep
- BBa_J37033(RBS+LuxR)
- BBa_J37020(Luxpr+LuxR+LuxR+GFP)
- BBa_J37032(pLux+GFP)
Digestion
- BBa_I7100(ptetR+RBS)
- BBa_S03119(ptet+LuxR)
- Ligation product(ptet+LasR)
- Ligation product(ptet+RhlR)
- Ligation product(ptet+cinR)
| Sample No | each |
| DNA template | 25 |
| EcoRⅠ | 1 |
| PstⅠ | 1 |
| BSA(x10) | 4 |
| EcoRⅠ Buffer | 4 |
| dH2O | 5 |
| TOTAL | 40 |
-->37℃ 1hour
->Next day
