Team:Chiba/Calendar-Home/26 September 2008

From 2008.igem.org

Revision as of 09:07, 24 October 2008 by Yoshimi (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

>Home | Notebook

25 September 2008 <|> 27 September 2008

Laboratory work

Team:Communication

Team:Output

TIME DELAY TEST

colony PCR
Ligation product(26,September)x7
Vector:BBa_R0062

Sample No	each
culture	1
Fwd primer	1.5
Rev primer	1.5
Taq(DNA pol)	0.2
dNTPmix	3
thermo pol Buffer	3
dH2O	19.8
TOTAL	30

->95℃ 5min ->(95℃ 1min 50℃ 30sec　72℃ 1min)x25 ->72℃ 10min
->Gel cheack
->MINI Prep

Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/26_September_2008"