Preparation of BioBricks

Michał K.

1. Digest of Z-GeneArt and RFP(????)+deltaA plasmids with NdeI and BcuI (BamHI buffer).
2. Digest of RFP(????)+deltaA plasmid with NdeI and SacI (BamHI buffer).
3. Gel electrophoresis of digested plasmids and gel-out of proper bands (Z - 200 bp and both RFP (??????)'s - 2200 bp).
4. Gel electrophoresis to estimate concentration of purified DNA from previous day.
5. Overnight ligation of isolated DNA fragments: RFP(??????) + Z and RFP(????) + OmpA_link (from 18 September).

Piotr

Inoculation of plasmids received from iGEM HQs ( ).