NTU@iGEM Project Introduction Methodology Team Introduction Team background Notebook Dry Lab Introduction ODEs used Modeling Constructs Parameters used Deterministic Modeling Sensitivity Analysis Stochastic Modeling Wetlab Introduction Materials and Equipment Protocols Verification of Detection & Lysis System Our New Parts Characterization Introduction Standard Promoters Characterization of pLacI-GFP pLacI-GFP images and clips Characterization of pLsrA-YFP New characterization Proposal Parameter Estimation & Correlations Acknowledgements

Contents 1 Methodology 1.1 Abstraction 1.2 Decoupling 1.3 Standardization

Methodology

The NTU@iGEM team adopted a simple yet rigorous Engineering concept which can be broken down into three steps:

1) Abstraction

2) Decoupling

3) Standardization

Abstraction

In abstraction, the complexity of things was reduced by creating simple parts before developing these parts into higher complex devices and systems

Decoupling

In decoupling, each individual part was decoupled from the others, devising them without consideration for the other existing parts. Following which the fusion of different combination of these components formed a working device.

Standardization

In standardization, the Team hopes to establish standards that could be widely used and agreed. We also try to predict the function of components and compatibility of parts leading to a set of standards and protocols that could be used for future teams.