Team:Warsaw/Calendar-Main/31 July 2008
From 2008.igem.org
Purification of proteins: Z-alpha and Z-omegaPiotr, Emilia
Cloning of omega_A DNA fragment to pACYC177+OmpA_alphaMichał K.
Cloning of truncated fragment of protein AMichał K.The arrow shows place of our overexpressed protein: 1. 22°C 0,5 mM IPTG 2. 22°C 0,1 mM IPTG 3. 22°C 1 mM IPTG 4. Marker 5. negative control 6. 37°C 0,1 mM IPTG 7. 37°C 0,5 mM IPTG 8. 37°C 1 mM IPTG
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