Team:Warsaw/Calendar-Main/2 October 2008

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Preparation of BioBricks

Michał K.

  1. Isolation of plasmid from culture inoculated on previous day (RFP(?????)+Z).
  2. Control digest of isolated plasmid with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.
  3. Digest of pLac_OmpA_omega fragment with EcoRI and BcuI (BamHI buffer).
  4. Gel elctrophoresis and gel-out of proper band - 1200 bp.
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