Preparation of constructs with OmpA protein fusions

Michał K.

1. Colony PCR with OmpaL_N and OmpaP_link primers on colonies from plates with transformations OmpA_alpha (annealing temperature - 55°C,45 s of elongation step).
2. Gel electrophoresis.
3. Confirmed transformant colonies inoculated to liquid LB with kanamycin.

Preparation of construct pKS with A protein

Michał L., Ewa, Marcin

1. Colony PCR on colonies from plates with transformations pKS-A.
   Primers used: AL+SacI and AP+NotI
2. Gel electrophoresis.
3. Confirmed transformant colonies inoculated to liquid LB with ampicillin.

Cloning of protein Z DNA to pET15b-OmpA-alpha in place of OmpA

Piotr, Antoni

Isolation of plasmids from cultures inoculated on previous day.