Team:Warsaw/Calendar-Main/29 August 2008

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Cloning of protein A DNA to pET15b+Omp-alpha plasmid

Michał K.

Isolation of pET15b+A-alpha plasmids.
Control digest of isolated plasmids with NdeI and NotI (Orange buffer).
Gel elctrophoresis. Chioce of proper clone.

Protein purification: A-alpha

Piotr

Single transformations of Rosetta with plasmids pET15b+A-alpha. Plating on LB with chloramphenicol and ampicillin.

Blue-white screening and rifampicin test in

Michał L.

GM2163 competent cells carrying pZC320 were transformed with following plasmids:

Transformants were selected on CTAXI plates (LB + Chloramphenicol + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal) because GM2163 are chloramphenicol resistant.