Team:Tokyo Tech/Construction

For checking pressure-response ability of Plac, we constructed three plasmids.Those are showed at figure 1.Plac stands for a promoter repressed by lacI protein. Ptet stands for promoter repressed by tetR protein.∆p stands for promoter less.JM109 is constitutive expression lacI protein so that Plac is always repressed by lacI protein.And JM109 isn’t expression tetR. Therefore, we used Ptet as a positive control.

Fig1

Each parts is constructed on low-copy plasmid “pSB6” ,and inserted into E.coli strain JM109.