"
Team:Tokyo Tech/Acrylic container
From 2008.igem.org
| Home | Construction | Acrylic container | Development of promoter | Genetic toggle switch | Parts Submitted to the Registry | Result | The Team | Notebook |
|---|
2. Acrylic container
 |
|
We create devices for confirming pressure response of lac promoter. This is the first step of creating touch display. This device made of Acrylic glasses and has two holes (show figure). Each hole contains tubes and water. Inside tubes E. coli is cultivated. Pressure can travel to inside tubes. One hole (A) is covered with a plastic tape (show figure). Therefore the hole is pressurized.The other (B) is covered with a block made of an acrylic glass. (show figure) Therefore the hole is not pressurized by water. |
|
E.coli type in tubes |
 |
* “Plac” stands for a promoter repressed by lac protein. On pSB6 plasmid (E.coli strain; JM109) |
|
After pressurized the container, we observed the E.coli by a fluorescence microscope. The result shows below. |
 |
 |
Our equipments for pressure experiments
Dilute this culture medium by 1% by adding fresh medium and suitable antibiotic (ampicilin; 50㎍/ml). Next, seal this culture medium with oxygen-saturated fluorinert (25% volume of medium) in polypropylene tubes with parafilm. (image1) Put this tube into pressure vessel filled with water. (image2) Next cup pressure vessel.(image3) Finally, pressurize pressure vessel by pressure device(image4). and then incubation was started at 37℃ immediately at each pressure vessel(original designed) for 16h at 37℃. After cultivation, the cells were examined by fluorecence microscope.
