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Team:Chiba/Calendar-Home/8 September 2008
From 2008.igem.org
7 September 2008 <|> 9 September 2008
Contents |
Laboratory work
Team:Input
no work
Team:Communication
-->(7/9)
- --->Zymo Clean
- eluted with 15μL(?) of NFW.
Team:Output
- [http://partsregistry.org/Part:BBa_R0040 BBa_R0040]①
- [http://partsregistry.org/Part:BBa_R0010 BBa_R0010]⑤
| Sample No. | ① | ⑤ | |
| DNA(digested sample)(μL) | 7 | 6 | |
| SAP(μL) | 2 | 2 | |
| SAP Buffer(μL) | 3 | 3 | |
| TOTAL(μL) | 30 | 30 |
-->left at 37 degrees for 1 hour.
-->left at 65 degrees for 30 minutes.
-->DNA purification(①:21μl,⑤:6μl)
-->Gel check
| Sample No. | each |
| DNA(digested sample)(μL) | 1 |
| Loading Dye(μL) | 1 |
| dH2O(μL) | 4 |
| TOTAL(μL) | 6 |
- vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]① 75ng/μl
- insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158]② 33.6ng/μl
- insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156]③ 33.6ng/μl
- insert:[http://partsregistry.org/Part:BBa_S03160 BBa_S03160]④ 38.4ng/μl
- vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]⑤ 100ng/μl
- insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]⑥ 33.6ng/μl
| Sample No. | ①&② | ①&③ | ①&④ | ① | ⑤&⑥ | ⑤ |
| DNA(vector)(μL) | 3 | 3 | 3 | 3 | 5 | 2.1 |
| DNA(insert)(μL) | 3 | 3 | 3 | - | 3.1 | - |
| Ligase(μL) | 1 | 1 | 1 | 1 | 1 | 1 |
| Ligase Buffer(x5)(μL) | 2 | 2 | 2 | 2 | 2 | 2 |
| dH2O(μL) | 1 | 1 | 1 | 4 | 8.9 | 4 |
| TOTAL(μL) | 10 | 10 | 10 | 10 | 20 | 10 |
