Team:Chiba/Sender experiments

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Project Design ( Sender 実験 | レシーバー実験 ) Making time delay | Our Goal

Quorum-Sensing Cross-talk

  • Senders
    • [http://partsregistry.org/Part:BBa_K084007 plac+rbs+LasI]

Tet-lasi chiba.gif

    • [http://partsregistry.org/Part:BBa_K084008 plac+rbs+RhlI]

Tet-rhli chiba.gif

    • [http://partsregistry.org/Part:BBa_K084012 plac+rbs+LuxI]

Tet-luxi chiba.gif

  • Receiver
    • [http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)]

Reporter 9002 chiba.gif


Method

To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.

  1. Transformed Senders into E.coli strains(JW1908/XL10GOLD) and Receiver into E.coli strain(JW1908).
  2. Inoculated them independently in liquid media. Incubated at 37℃ 12h
  3. Mixed them.
  4. Incubated at 37℃ or 30℃.
  5. Measured intensity of green fluorescence at regular time intervals.

Result

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